Determination of pesticide residues in wine by solid-phase extraction on-line combined with liquid chromatography tandem mass spectrometry

Liquid chromatography tandem mass spectrometry (LCMS/MS) has been combined with different sample preparation strategies to determine potential pesticide residues existing in wine. Herein, we propose the on-line coupling between solid-phase extraction (SPE), based on a reversed-phase polystyrene-divinyl benzene (PS-DVB) sorbent, and LCMS/MS for automated monitoring of selection 48 pesticides (37 fungicides 11 insecticides) this beverage. Parameters affecting sensitivity, selectivity, accuracy robustness method are evaluated as function volume, clean-up conditions, number extraction-desorption cycles performed same SPE cartridge. Considering volume 3 ?L, was free MEs, attaining LOQs below 2.5 ?g L?1, providing recoveries range from 70 120 % 90 investigated compounds. Increasing loaded cartridge 25 ?L permitted further reduction LOQs, linear response (0.1–25 L ?1), covering analysis low residue samples. Twelve out compounds were noticed at individual concentrations above 10 L?1 least one 30 processed wines. In white wines, sum iprovalicarb, fenhexamide metalaxyl represented more than 50 total residues. As regards red tebuconazole, cyprodinil predominant species.